
After a few days of observation, Kuroto was able to confirm that the two pairs of Mangekyou Sharingan in Ryota's eye socket had started the initial fusion, but the fusion speed was very slow.
Standing in front of the test bench, Kuroto held her chin with her right hand and her left hand on the chest*thinking posture** and muttered to herself, “At this speed, she said, both pairs of mangekyo sharingan will take at least three months to fully fuse into the Mangekyou Sharingan Abadi.”
Ryota, the trigger that does the fusion, is in a coma, so the fusion of two pairs is not done with his will like a voluntary process, but rather occurs in his subconscious.
Therefore, the merger of the two Mangekyou Sharingan pairs into the Eternal Mangekyou Sharingan has slowed down.
And Kuroto seemed to have no effective solution to solve this problem and had to wait quietly.
As for whether the two pairs would actually successfully fuse together or which technique the Eternal Mangekyou Sharingan would use, Kuroto no longer had a say in this, it all depended on the end result.
So, putting aside the matter of the Eternal Mangekyou Sharingan for now, Kuroto would start the cultivation plan of the Flame Realm Clone.
The difficulty of Cultivating a Flame Realm Clone was undoubtedly much higher than the previous Water realm Clone.
This was not only because the Flame Realm was extremely ferocious and searing and therefore unstable like the Water Realm but also because the subject whose genes Kuroto had chosen for the Flame Realm Clone was a member of the Clan Uchiha.
Although the Uchiha Clan was born with a natural affinity for the Fire Element, it was still considered a secondary chakra, while the Yin Properties were the Chakra Traits that were more commonly owned by members of the Uchiha Clan, and Kuroto also has a very dense and powerful Yin. Chakra, which symbolizes spiritual energy and is required for the Kekkei Genkai Dojutsu type.
Thus during the entire cloning process, Kuroto not only had to filter out his own Yin Chakra, but also the Yin Shisui Chakra to make the Fire Chakra realm the main trait of the resulting clone, he said, otherwise clones would be a waste of effort and resources.
Kuroto already has some experience in filtering out the Yin Cakra as he filters his own Yin Cakra while creating the Water Realm Clone, which is the, he therefore understood that the process of filtering the Natural Chakras causes some degree of damage to the cell.
At that time, only Kuroto's cells needed to be filtered while Yuki Haku's cells were being used as they were, but this time his and Shisui's cells had to be filtered.
In other words, the cell damage will be higher, which will reduce the chances of successful smelting of both types of cells.
Buzzing…
At this time the alarm from the Large Centrifuge filter sounded indicating that the sample of cells he placed in the centrifuge filter for the filtration of the Yin Chakra from both his cells and Shisui cells had been completed.
Initially, Kuroto prepared 500 samples from his cell. Since Kuroto already had a certain level of experience in filtering the Yin Chakra from his cell, therefore, he had a better understanding of which potential was used to not damage the cells too much. As more samples survived the screening process than the last time, the number reached 89!
True, the number of samples that survived the filtration process was 89.
As for the rest?
They are either too damaged or completely lose their cell activity.
Similarly, Kuroto prepared another 1000 samples from Shisui's cells. He prepared twice the number because this was his first time, he did the Chakra filtration for the Uchiha cells, he said, therefore he had to work through various potentials and find which one matched the sample of the cell perfectly, again this was manual work and took many days.
Finally, the number of surviving samples was 73.
A total of 89 and 73 samples are considered feasible to be processed and used for the next stage of the cell fusion process while the rest is discarded and discarded.
Without further ado, Kuroto begins the process of cell fusion
Similar to last time, the ratio of the number of cells he used for fusion was 99:1.
In simple words, for every Shisui cell, 99 of its cells are used.
That is, the peak cell that will be used to make cell buds for the cultivation of the Flame Realm Clone will have 99% of Kuroto cells and 1% of Shisui cells.
For those wondering how is it possible, because the number of cell samples does not match this ratio?
Before we talk about the number of cell samples, not the number of cells, each sample contains more than one cell so keeping the ratio is very easy.
The number of final samples that went through the fusion process was 73.
After injecting Shisui's cells into 73 samples one by one, Kuroto quietly waited for the fusion process to begin.
Immediately, failed results begin to appear, which means that the fusion of cells does not occur, and resulting in rejection, there may be several causes for this, which include, insufficient cell activity, Mismatched DNA, cell death that fused in the middle of the path.fusion process and so on.
In less than an hour, the fusion reaction of 59 samples resulted in failure.
In the next few minutes, the remaining 14 samples also began to lose cell activity, by the time the entire fusion process was completed for all the samples, only one sample had survived the fusion process, while the rest were all dead.
Kuroto who was observing the entire fusion reaction had a serious expression on his face.
At the time of cloning the Suijin Clone, the number of samples that went through the fusion process was only 6, and even of those 6, 3 samples managed to survive the fusion process, showing a 50% success rate%.
But this time, 73 samples went through the fusion process, and still only 1 sample survived the fusion process, the success rate of the experiment was not even 1.37%.
"Does the Hyuga and Uchiha DNA combination have such a high rate of rejection?"
Although Kuroto hoped that the cultivation of the Flame Realm Clone would not be easy, and would require repeated trials and errors even during the cloning process, he said, he did not expect that the success rate of the fusion process would drop to a very low percentage.
Sighing slightly, Kuroto collected the only sample that survived the fusion process, named it Sample-1A, placed it on a stage clip of a high-precision microscope, and observed it through the Eyepiece.
While observing the group of fused cells, Kuroto's face was filled with disappointment.
This is because the only samples that survived the fusion process also suffered very severe damage, and the cell activity was also very low, so it does not conform to the minimum standards required to create a cloned body and is therefore classified as unsuitable.
But Kuroto thought a little and after a moment of hesitation, Kuroto decided not to throw it away but put it into an incubator to begin development.
In the weeks that followed, Kuroto continued this experiment, Chakra filtration, fusion, and so on, and the results were much worse than he expected, and the results were much worse than he expected, no matter how many samples were used or how many samples went through the fusion process, none of them were successful.It is considered successful, any reaction to the fusion process results in failure!
This became the reason for Kuroto's distress, even after spending so much time, money, energy, and energy, the result he obtained was repeated failure, finally, he couldn't help but wonder, “Is it possible that my genes and Shisui are incompatible? that fusion process produces all but failure?”
This doubt was not unreasonable, the success rate of the previous fusion experiment reached 50%, but the overall success rate this time did not even reach 0.1%, either%, and the gene mismatch was the only reason that Kuroto could only think of for such a thing.
And strangely, as Kuroto continues her experimental process, the cloned buds made from Sample-1A (which Kuroto considered unsuitable but not discarded) and placed in the Big nutrient tank to undergo the cultivation process were very lucky to be able to thrive.
However, Kuroto would always frown whenever she observed this burgeoning Clone.
Since Kuroto could not understand why this mismatched sample had undergone some sort of change as a result, her gender changed to female as it progressed from stem cells to its current state, completely deviating from Kuroto's plan.
"
"
Of course, Kuroto was unable to accept the use of this female clone, so she had to continue cloning experiments, with different cell samples including, Itachi, Shinichi, etc.
Now, Kuroto no longer felt that she had so many options to choose from, because the rejection between the Uchiha and Hyuga genes was extremely high!
.
.