I Have Genius DNA
Eps 8 Reverse Deferential Stem Cells (2)

"That's right. That's the real Nobel prize." Jung Hye-rim agrees.

"Even if it is different from our department's work, they can hardly find fault at the seminar at the home of the head of the research center." Park Dong Hyun said.

Jung Hye-rim nodded and added. "Once a cell differentiates, it cannot reverse. It's like we can't go back to being a scholar anymore because we're already doctors."

Park Dong-hyun returns to his presentation. "Anyway, we focus on artificial cell synthesis. Let's take a look at this. Let me tell you what happened."

Ryu Young-joon stared at the flashing message window before his eyes.

Rosalin said it was possible. What progress would happen if this technology was secured?

No need to collect eggs, no need to collect *****, no need to create fertilized eggs. There is no need for micro-control with very high levels of difficulty to replace genetic material.

Therefore we only take the patient's own cells, it will be no rejection. This is a leap from the time when women used to scrub laundry with washing boards in the river every day to the era of sophisticated drum washers.

Clik.

Ryu Young-joon presses Rosalin's message window.

"What are you doing?" Ko Soon-yeol tilts her head when she sees Ryu Young-joon pointing his finger in the air.

"Oh, in my hand is dust." Ryu Young-joon makes an unclear excuse.

Fitness Rosaline, which is currently owned by Ryu Young-joon is 1.5. This is the maximum.

To get higher, I have to raise Rosalin's level. But how?

Ting..!

I was thinking about it, but suddenly the message window appeared with an alarm sound.

, improve transfer status or synchronization status.>

These are the messages I read a long time ago. This is also information I already know. The question is how to improve transfers or synchronization.

Ryu Young-joon confirmed Rosalin's current status.

Transmission status:

Heart (2%)

Liver (46%)

Kidneys (13%)

Spinal cord (4%)

These values are the result of Rosalin's efforts to recover her body when Ryu was an alcoholic. He has cured all sorts of ailments such as lumbar disc, turtle neck, and fatty liver.

Why don't you try some cyanide?' I imagined something ridiculous and I immediately focused on listening to Park Dong-hyun's voice, who was leading the presentation.

"The latest test 4.87 was destroyed because the cell membrane is unstable."

4.87!

Ryu Young-joon's eyes are wide open. Apparently, when Rosalin was first obtained, the artificial cells that responded in the lab were labeled v4.87. It was a collection of chemicals that could not yet be called living things, but Rosalin was born when Ryu Young-joon's blood got into it. What happened then?

ATP from my blood cells goes into v4.87.

Maybe ATP is an important factor for Rosalin growth?

ATP is a very high-energy chemical that is widely used in the metabolism of all cells in the body. And Rosalin, who is now in Ryu Young-joon's body, is at least reacting heavily to ATP.

So if I put ATP into it, will it increase the transfer or synchronization state?

"Do you have a good idea?" ask Park Dong-hyun.

However, both Jung Hye-rim and Ko Soon-yeol just duck to avoid eye contact.

Park Dong-hyun sighed softly.

"It's okay, not because you're bad so we don't make progress on our project. This project itself is a very unrealistic and difficult task."

"How long does the end-of-year report last?" ask Ryu Young-joon.

"two weeks or more......."

Ryu Young-joon sank into his thoughts for a moment. This is not a bad project. If embryonic stem cells could be produced from normal cells, they would have a huge impact.

If such an announcement is made, Ryu Young-joon's name will soon become a star in the scientific community. This is the best condition to increase its presence in the company. Another advantage is that it is the underlying technology.

Success doesn't end there. Subsequent studies of high levels of difficulty, such as culture of artificial organs, tissues, and neuro-differentiation are needed. There is no hard task for modern scientists. The next key remained in Ryu's hands, so he could take the initiative.

After the release of the data, I will need some manipulation, but I can do well. If this works out, the relationship between me and the company will be good. The first author of the paper will also occupy the first inventor and the maximum part of the patent. Ryu Young-joon looks at Park Dong-hyun, Jung Hye-rim and Ko Soon-yeol calmly.

"I'll study the inverse differentiation of stem cells during that time."

"That's not allowed." Park Dong-hyun shows her displeasure.

"But I think it's okay to let them do whatever they want because he's new" Ko Soon-yeol said. Park Dong-hyun nodded after a short thought.

"Okay, we have to successfully make a year-end report, so we're not in a precarious situation doing new work, and Dr. Ryu needs time to learn. Do what you want to do for two weeks...."

"Thank you."

After a while, the meeting ended. No useful ideas were obtained for the year-end report. But it doesn't matter. Ryu Young-joon is convinced that he will make it himself. As soon as Ryu returns to the lab, he searches for ATP.

Found her.

He took out a small liquid reagent bottle from the freezer-20 degrees.

This is ATP (Adenosin 5'-Tryphosphate) by removing less of the sodium and only reducing the front. Ryu Young-joon extracts about 1 milliliter of ATP from the reagent bottle.

Strain into a 0.22-micrometer filter, sterilize it, then put it in a 5-milliliter syringe in the laboratory. Alcohol disinfects the inside of the elbow and injects ATP intra-venously. I can't believe it's time for my body's clinical trials. To be honest, that's crazy. However, this dose is clearly not harmful to the body of a healthy person. Even if Rosalin had no medicinal effect, it would end up without damaging Ryu's body.

'Let's see.....' Ryu Young-joon leaves the window of Rosaline's status open and keeps an eye on her.

Tings....!

After a while the synchronization level or transfer status did not go up? "Isn't this?" Ryu Young-joon was about to see the status window but only improved the fitness of the cells temporarily.

Rosaline Lv.1

Transmission status :

Heart (2%)

Liver (46%)

Kidneys (13%)

Spinal cord (4%)

Synchronization: 3%

Cell fitness: 2.1 (+0.6 by ATP, for 21 hours)

Control of genetic expression :

CYP2E1B (38%)

Fitness items have value (+0.6/21

clock, by ATP).

Looks like that means it will add 0.6 for 21 hours. After all, the total amount of fitness was more than 2. Ryu Young-joon quickly reopened the message window.

Clik.

I pressed the button with my finger and before long the solution appeared. By manipulating some of the genes in a normal cell, I was able to reverse the state of embryonic stem cells. In the simplest way that I can use right now, Rosalin recommends 4 sets of genes :

SOX2, cMyc, OCT4, F4.

Overexpressing the four abdominal genes in the cells.

'Ugh...' I felt like I was looking at the answer sheet of God that suddenly appeared. Who could have known to quickly manipulate all four simultaneously of the 20,000 genes initializing a normal cell into an embryonic stem cell?

If I were to pick a gene and experiment randomly, this knowledge would likely win the first prize hundreds of millions of times. I goosebumps again because Rosalin knows all the winning numbers. Ryu Young-joon runs straight to the office computer and closes the message window.

I searched for genebank.com in the address bar and logged in with Ryu's ID. This is the place to sell genes. Artificially synthesized genes are sold separately for each type. When ordered, I was able to obtain the frozen and dried genes in micrograms, dissolve them with water, and use them in experiments. Ryu Young-joon immediately ordered the four types of genes.

***

The gene arrived the next day, but his experiments still needed more detail.

How many genes are inserted into a cell in what way? And How long after entering it will be observed?

Initially, Ryu Young-joon will spend at least a month at each step, setting the conditions one by one, but Ryu does not have to. Synchronization mode also provides optimal conditions for experimentation.

If I use fitness carefully, I can get the following message.

Ryu Young-joon conducts the experiment Following the instructions shown by Rosalin, consuming fitness every day. By multiplying human embryonic kidney cells, the gene is placed in a retrovirus and reproduced.

This experiment lasted for several days.

And on Friday morning. Ryu eventually infects human embryonic kidney cells with the virus. Now, the four genes that Ryu Young-joon, SOX2, cMyc,OCT4 and KLF4 enter will begin to be expressed in these cells.

Now all I have to do is watch. If this cell is initialized in the state of a fertilized egg, it is successful. By the way, it's very simple.' Compared to cooking, what scientists do is collect ingredients that were originally unknown and create new recipes.

But following Rosalin's instructions is like cooking with recipes and prepared ingredients. The difference in the level of difficulty is far and wide. Ryu ends up putting genetically modified human embryonic kidney cells on a special STO culture plate.

If these cells differentiate as they grow here, they can be called "artificial embryonic stem cells". Is possible?

While observing the cells from the breeding plate under a microscope, Jung Hye-rim talks to her from behind.

"What are you doing?" He approaches Ryu Young-joon and looks at the medium.

"What's this?"

"Hand cell candidate."

"Oh."

"This is the stem cell I want to grow. I'll send you detailed special detail trial by email."

"I hope it works, but I honestly didn't expect it."

"It's lunchtime, don't you eat?"

"I'm leaving now. I'm here to ask you to eat with me. Let's go."

Following Jung Hye-rim, Park Dong-hyun and Ko Soon-yeol are already waiting in front of the elevator with their coats.

"I'm going to the cafeteria, so do you have to wrap your coat like that?" ask Ryu Young-joon.

"The hallway is cold too. And I had to queue long enough to get a meal ticket, so I put on my coat" Jung Hye Rim said.

"I hear the flu is going on lately. If it's cold, you'll lose your immunity, so be careful." Park Dong Hyun said.

"Huck....Flu?" Jung Hye-rim shivers.

"Yes, take your vitamins. And Dr. Our Ryu Team always eats together, if possible." Park Dong Hyun said.

"Thank you?"

"Yes, I told you before, shouldn't we be friends with each other. Otherwise, I wouldn't be able to stand here."

Jung Hye-rim and Ko Soon-yeol laughed.

Park Dong-hyun continued to say. "I can't join Dr. Ryu this week because he's busy experimenting at lunch, but let's do it together next week."

Ryu Young-joon nodded.

We've talked about eating together a few times during the week. However, Ryu Young-joon refuses. The trial schedule must be strict to produce significant results within two weeks.

Now that my busy schedule was over and I could wait for the results to come out, I packed some food for a few days and had lunch and went straight to the cafeteria, upon arriving at the cafeteria Ryu Young-joon queues to pick up his food ticket.

It sounded!

Ryu Young-joon's profile appears on a small monitor when he takes a photo of his employee's card to get a meal ticket.

Ryu Young-joon, the principal investigator of the Department of Life Creation At the same time, I could feel a strange sting from the eyes of the scientists lined up behind me.

"Hey, why are you in lab six?" Someone whispered behind me.

"At a report later this year, the lab's performance was down. One research center operated by Director Kim Hyun-taek directly led a new anti-cancer drug team to a percentage due to the events that befell him"

"I'm sure nothing happened this time, right?"

"The atmosphere at the end-of-year seminar will be broken again. Always awkward after a team presentation."